Development of a molecularly imprinted polymer (MIP)-based optical sensor for the detection of macrolide antibiotics: eva‎luation of isotherm an‎d kinetics

5/9/2027 12:00:00 AM

The increasing occurrence of azithromycin (AZI) residues in environmental an‎d food samples, together with growing concerns over antimicrobial resistance, necessitates the development of rapid, sensitive, an‎d selec‎tive analytical platforms. A multifunctional colorimetric sensing system based on a Fe₃O₄/ZIF-8 nanozyme integrated with a surface molecularly imprinted polymer (MIP) was developed for the selec‎tive detection of AZI in aqueous media. The structural, magnetic, an‎d textural properties of the synthesized nanocomposite were confirmed using multiple analytical techniques. The resulting Fe₃O₄/ZIF-8@MIP exhibited a high adsorption capacity of 191.32 mg·g⁻¹ with an imprinting factor (IF) of 3.16, an‎d followed the pseudo-first-order kinetic model (R2=0.9895) while fitting the Langmuir isotherm model (R2=0.9931). The composite displayed strong peroxidase-like activity, catalyzing the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) by H₂O₂ to generate a blue colorimetric signal. By optimizing polymerization parameters an‎d sensing conditions, the sensor provided a linear response to AZI from 1–150 µM, with a limit of detection (LOD) an‎d quantification (LOQ) of 0.13 µM an‎d 0.44 µM, respectively. Briefly, the colorimetric sensor had good stability, selec‎tivity, an‎d feasibility for detection of AZI in the presence of different antibiotics an‎d interfering ions an‎d organic substances.

سنجش آنتي‌بيوتيك‌ ماكروليد , پليمر قالب ‌مولكولي , چارچوب ايميدازولي زئوليتي-8 , اكسيداسيون TMB , حسگر رنگ¬سنجي

Macrolide antibiotic detection , molecularly imprinted polymer , zeolitic imidazole framework-8 , TMB oxidation , colorimetric sensor

Fatemeh Aminian Malekabadi

Dr. Alireza Hemmati